Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Immunofluorescence, Flow Cytometry, Staining

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Flow Cytometry, Staining, Control, Incubation, Inhibition

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Starch

Journal: Digestive Diseases and Sciences

Article Title: LeLISA: A New Lectin-Based Immunoassay for Evaluation of Mucinous and Serous Content in Pancreatic Cystic Neoplasms

doi: 10.1007/s10620-025-09188-4

Figure Lengend Snippet: LeLISA testing of cyst fluids from 10 pancreatic mucinous cysts (Muc) against eight different lectins. Wells were coated with lectins, 200 µg/ml in TBS, 50 µl per well, for 18 h. Cyst fluids were diluted 1:6 in TBS with Sigma Fast protease inhibitor mix; incubated at RT for 60 min followed by 60 min with HRP-conjugated monoclonal anti-Cp followed by 20 min with substrate. PNA Peanut agglutinin, Lens Lens culinaris agglutinin, WGA Triticum vulgaris lectin, Galant Galanthus nivalis lectin L, Narc Narcissus pseudonarcissus lectin, Jacal Artocarpus integrifolia lectin, Phaseol Phaseolus vulgaris lectin, Agar Agaricus blazei Murill

Article Snippet: The following lectins were purchased from Medicago AB, Uppsala, Sweden: Arachis hypogaea lectin (PNA), specificity: Galβ1-3GalNAc; Lens culinaris lectin-LCA, specificity: Core-fucose N-glycans; High-mannose N-glycans.

Techniques: Protease Inhibitor, Incubation

Journal: Digestive Diseases and Sciences

Article Title: LeLISA: A New Lectin-Based Immunoassay for Evaluation of Mucinous and Serous Content in Pancreatic Cystic Neoplasms

doi: 10.1007/s10620-025-09188-4

Figure Lengend Snippet: Binding of HRP-anti-Cp when 10 pancreatic serous cyst fluids (Ser CV) were incubated in lectin coated wells. Eight different lectins, 200 µg/ml in TBS, were used, 50 µl per well, for 18 h. Cyst fluids were diluted 1:6 in TBS with Sigma Fast protease inhibitor mix, incubated at RT for 60 min followed by 60 min with HRP-conjugated monoclonal anti-Cp and for 20 min with substrate. PNA Peanut Agglutinin, Lens : Lens culinaris agglutinin; WGA Triticum vulgaris lectin, Galant Galanthus nivalis lectin L, Narc Narcissus pseudonarcissus lectin, Jacal Artocarpus integrifolia lectin, Phaseol Phaseolus vulgaris lectin, Agar Agaricus blazei Murill

Article Snippet: The following lectins were purchased from Medicago AB, Uppsala, Sweden: Arachis hypogaea lectin (PNA), specificity: Galβ1-3GalNAc; Lens culinaris lectin-LCA, specificity: Core-fucose N-glycans; High-mannose N-glycans.

Techniques: Binding Assay, Incubation, Protease Inhibitor

Journal: Digestive Diseases and Sciences

Article Title: LeLISA: A New Lectin-Based Immunoassay for Evaluation of Mucinous and Serous Content in Pancreatic Cystic Neoplasms

doi: 10.1007/s10620-025-09188-4

Figure Lengend Snippet: Binding of HRP-anti-Cp when 10 pancreatic pseudocyst fluids (Ps CV) were incubated in lectin-coated wells, eight different lectins, 200 µg/ml in TBS, 50 µl per well, for 18 h. Cyst fluids were diluted 1:6 in TBS with Sigma Fast protease inhibitor mix and incubated at RT for 60 min followed by 60 min with HRP-conjugated monoclonal anti-Cp followed by 20 min with substrate

Article Snippet: The following lectins were purchased from Medicago AB, Uppsala, Sweden: Arachis hypogaea lectin (PNA), specificity: Galβ1-3GalNAc; Lens culinaris lectin-LCA, specificity: Core-fucose N-glycans; High-mannose N-glycans.

Techniques: Binding Assay, Incubation, Protease Inhibitor

Journal: Digestive Diseases and Sciences

Article Title: LeLISA: A New Lectin-Based Immunoassay for Evaluation of Mucinous and Serous Content in Pancreatic Cystic Neoplasms

doi: 10.1007/s10620-025-09188-4

Figure Lengend Snippet: LeLISA with Galanticus lectin and HRP-conjugated anti-Cp. Optical density (OD) values for 10 cystic fluids of each type: serous cysts, pseudocysts, and mucinous cysts; means and 95% confidence intervals

Article Snippet: The following lectins were purchased from Medicago AB, Uppsala, Sweden: Arachis hypogaea lectin (PNA), specificity: Galβ1-3GalNAc; Lens culinaris lectin-LCA, specificity: Core-fucose N-glycans; High-mannose N-glycans.

Techniques: